Part 1 – Sampling from the Ballast Water Discharge Line
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Statutory Documents - IMO Publications and Documents - Resolutions - Marine Environment Protection Committee - Resolution MEPC.173(58) – Guidelines for Ballast Water Sampling (G2) –(adopted on 10 October 2008) - Annex – Guidelines for Ballast Water Sampling (G2) - Annex - Part 1 – Sampling from the Ballast Water Discharge Line

Part 1 – Sampling from the Ballast Water Discharge Line

  1 The advantage in sampling the biota present in the ballast water discharge line is that this is most likely to accurately represent the concentration of substances and organisms in the actual discharge, which is of primary concern in assessing compliance with the discharge regulations.

  2 The disadvantages of this method are that, on most ships, in-line sampling should be carried out in the engine room, where space may be limited, and the handling of water once the samples were concentrated may be impracticable.

  3 In order to undertake an accurate measurement on the organism concentration in the ballast water, it is recommended to install an “isokinetic” sampling facility. Isokinetic sampling is intended for the sampling of water mixtures with secondary immiscible phases (i.e. sand or oil) in which there are substantial density differentials. In such conditions, convergence and divergence from sampling ports is of significant concern. Since most organisms are relatively neutrally buoyant, true isokinetic sampling is unnecessary. However, the mathematics related to isokinetic sampling are deemed to be useful as a basis for describing and specifying sampling geometries. Isokinetic sampling is necessary to ensure that a sample contains the same proportions of the various flowing constituents as the flow stream being sampled. During isokinetic sampling the sampling device does not alter the profile or velocity of the flowing stream at the moment or point at which the sample is separated from the main flow stream. Under isokinetic conditions, the velocities of both the sample and the main flow are equal at the point at which the sample is separated from the main flow. To achieve isokinetic sampling conditions, a sampler is designed to separate a subsection of the total flow-stream in a manner that does not encourage or discourage water entry other than that which is otherwise in the cross-section of the sampler opening. In other words, flow streams in the main flow of the pipe should not diverge or converge as they approach the opening of the sampler.

4 Technical specifications for design of in-line sampling facilities

  4.1 Through computational fluid dynamics modelling, it has been shown that the isokinetic diameter calculation can provide guidance for sizing of sample ports for sampling of organisms.

 Simulations showed that flow transitions from the main stream were best for sample port diameters between 1.5 and 2.0 times the isokinetic diameter. Ports sized in this range had smooth transitions and pressure profiles that allowed for direct sampling without the need of a pump to induce sample collection. The isokinetic sample port diameter should therefore be determined generally according to the equation:

 where Diso and Dm are the diameters of the sample port opening and the main flow in the discharge line, respectively; and Qiso and Qm represent the respective volumetric flow rates through the two pipes. It is recommended that sample port size be based on the combination of maximum sample flow rate and minimum ballast flow rate that yields the largest isokinetic diameter.

  4.2 The opening of the sampling pipe should be chamfered to provide a smooth and gradual transition between the inside and outside pipe diameters.

  4.3 The length of the straight sample pipe facing into the flow can vary, but should not usually be less than one diameter of the sampling pipe. The sampling port should be oriented such that the opening is facing upstream and its lead length is parallel to the direction of flow and concentric to the discharge pipe which may require sampling pipes to be “L” shaped with an upstream facing leg if installed along a straight section of discharge pipe.

  4.4 The need to be able to service the sample pipe is important and should be considered, taking the safety of ship into consideration. Therefore, the sampling pipe should be retrievable either manually, or mechanically, or it should be in a system which can be isolated. Because of the potential for the opening and interior of the sample pipe to become occluded by biological or inorganic fouling, it is recommended that samplers be designed to be closable at the opening, removed between sampling intervals or be easily cleaned prior to sampling.

  4.5 The sample pipe and all associated parts of the sampler that come into contact or near proximity with the ballast piping should be constructed of galvanically compatible materials and generally corrosion resistant. Any corrosion of the sampling system will affect sample flow rates and potentially sample representativeness.

  4.6 If flow control of the sample flow rate is required, ball, gate and butterfly valve types should be avoided as they may cause significant shear forces which may result in organism mortality. For flow control, it is recommended that diaphragm valves or similar valve types be used to minimize sharp velocity transitions. For flow distribution, ball valves may be utilized in such a manner that they are either fully open or fully closed.

5 Technical specifications for installation of a sample point in the ballast water discharge line

  5.1 The sample taken should be removed from the main pipeline at a location where the flowing stream at the sample point is representative of the contents of the stream. The sample facility should be placed at a point where the flow in the main pipe is fully mixed and fully developed.

  5.2 The sampling point should be installed in a straight part of the discharge line as near to the ballast water discharge overboard as practicable. The sampling facility should be positioned such that a representative sample of ballast water is taken. It is recommended that the position of the sample point is established using methods such as computational fluid dynamics.


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