2.3.1 Regulation D-2 of the Convention refers
to two size categories of organisms and a group of indicator microbes.
Ships conducting ballast water management in accordance with regulation
D-2 shall discharge:
-
.1 less than 10 viable organisms per cubic metre
greater than or equal to 50 micrometres in minimum dimension;
-
.2 less than 10 viable organisms per millilitre
less than 50 micrometres in minimum dimension and greater than or
equal to 10 micrometres in minimum dimension; and
-
.3 discharge of the indicator microbes shall not
exceed:
-
(i) Toxicogenic Vibrio cholerae (O1
and O139) with less than 1 colony forming unit (cfu) per 100 millilitres
or less than 1 cfu per 1 gramme (wet weight) zooplankton samples;
-
(ii)
Escherichia coli less than 250
cfu per 100 millilitres; and
-
(iii) Intestinal Enterococci less
than 100 cfu per 100 millilitres.