1 The sample volume and number of samples required
will depend upon:
-
.1 the objective of sampling, e.g., to determine
the number of organisms in different size classes; to assess the viability
of organisms in different size classes; or to assess compliance with
the D-1 or D-2 standard;
-
.2 the specific analytical method to be used;
and
-
.3 the statistical significance and certainty
required.
2 Sample handling and storage will also vary depending
on the objectives and specific analytical methods. In particular the
way the sample is taken (e.g., net or pump) and the conditions in
which it is stored (e.g., light, temperature, storage container) should
be appropriate for the analytical method used.
3 Sample analysis methods are rapidly developing
and the best available procedures should be used consistently with
availability.
4 The sampling and analysis methodologies to test
for compliance with the Convention are still in development. Although
significant technical advances and refinements have been made in these
areas since the adoption of the Convention, there are still numerous
issues to be resolved. Administrations are still undertaking research
to define the most appropriate methods to test for compliance, and
the best way to take, handle and analyse samples.
5 At the present time, there are no specific sampling
or analysis protocols that can be recommended for Administrations
to use. However, it is expected that in due course this information
is likely to become available once full compliance testing regimes
are developed and Administrations have had time to gain experience
and develop best practice in ballast water sampling and analysis.
6 An IMO circular will be developed as a high-priority
matter, to provide sampling and analysis protocols to be followed
and give advice on the uniform application of these protocols. Such
a circular will be updated when new protocols are developed.
7 To aid this process, Administrations are requested
to supply information on any scientifically validated sampling and
analysis techniques to the Organization, as soon as possible.